PriMed has the GE Vivid S5 Ultrasound Machine on site to perform standardized echocardiography. All images were obtained by a clinical ultrasound doctor with >30 years of human ultrasound experiences and >2 years of NHPs ultrasound experiences. All data were analyzed by using GE EchoPAC software Only.

LA Volume, LA Volume Index

IVSd, IVSs, LVIDd, LVIDs, LVPWd, LVPWs

Systolic

1, LVEF (Biplane Simpson Method)

2, EDV, ESV, SV, FS, CO

3, Longitudinal/Circumferential Strain (Speckle Tracking)

Diastolic

1, E, A, E/A, A Dur.

2, e’, a’, e’/a’, E/e’, IVRT, IVCT

3, S, D, S/D, Ar, Ar Dur., Ar-A Dur.

4, Vp

PriMed use Siemens trio 3.0T MRI machine to perform cardiac magnetic resonance by an experienced team which lead by a clinical doctor with >30 years of human MRI experiences and >4 years of NHPs MRI experiences. Data were analyzed by CVI42 Software and Matlab Software.

EDV, ESV, SV, CO, LVEF

Peak systolic circumferential/longitudinal/radial strain (rate), Peak Torsion

ECV (cardiac fibrosis), Pre T1, Post T1, T2, T1rho, MBF

PriMed use Siemens SOMATOM Definition Flash CT Scanner to perform abdominal Dual-energy CT on rhesus to quantify liver fat content in NAFLD/NASH monkeys. Data were analyzed by Syngo dual-energy software.

Attenuation curve, Hepatic attenuation index (△HU_{Sn140-100kVp, HU100kVp/Sn140kVp})

Liver size, fatty liver, ultrasound-guided liver biopsy

Renal resistance index, ultrasound-guided kidney biopsy

Vessel plaque, atherosclerosis